Composite
Part:BBa_K1922012:Design
Designed by: WANG HONGNING Group: iGEM16_Tongji_Shanghai (2016-10-14)
hsp70-p53-luciferase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 8
Illegal PstI site found at 359 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 270
Illegal NheI site found at 2216
Illegal PstI site found at 8
Illegal PstI site found at 359 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2537
Illegal BamHI site found at 415
Illegal BamHI site found at 2369 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 8
Illegal PstI site found at 359 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 8
Illegal PstI site found at 359
Illegal NgoMIV site found at 2571 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 220
Illegal BsaI.rc site found at 1322
Illegal SapI site found at 404
Design Notes
It will optimize the efficiency of heat treatment.
Source
It comes from specific genomic sequence of our submitted basic parts.